Directly to content
  1. Publishing |
  2. Search |
  3. Browse |
  4. Recent items rss |
  5. Open Access |
  6. Jur. Issues |
  7. DeutschClear Cookie - decide language by browser settings

Sec61p is Adjacent to Nascent Type I and Type II Signal-Anchor Proteins during Their Membrane Insertion

High, Stephen ; Andersen, Soren S. L. ; Görlich, Dirk ; Hartmann, Enno ; Prehn, Siegfried ; Rapoport, Tom A. ; Dobberstein, Bernhard

In: The Journal of Cell Biology, 121 (1993), Nr. 4. pp. 743-750

[thumbnail of High_1993_JCB.pdf]
Preview
PDF, English
Download (1MB) | Terms of use

Citation of documents: Please do not cite the URL that is displayed in your browser location input, instead use the DOI, URN or the persistent URL below, as we can guarantee their long-time accessibility.

Abstract

We have identified membrane components which are adjacent to type I and type II signal-anchor proteins during their insertion into the membrane of the ER. Using two different cross-linking approaches a 37-38-kD nonglycosylated protein, previously identified as P37 (High, S., D. G6dich, M. Wiedmann, T. A. Rapoport, and B. Dobberstein. 1991. J. Cell Biol. 113:35-44), was found adjacent to all the membrane inserted nascent chains used in this study. On the basis of immunoprecipitation, this ER protein was shown to be identical to the recently identified mammalian Sec61 protein. Thus, Sec61p is the principal cross-linking partner of both type I and type II signal-anchor proteins during their membrane insertion (this work), and of secretory proteins during their translocation (G-tdich, D., S. Prehn, E. Hartmann, K.-U. Kalies, and T. A. Rapoport. 1992. Cell. 71:489-503). We propose that membrane proteins of both orientations, and secretory proteins employ the same ER translocation sites, and that Sec61p is a core component of these sites.

Document type: Article
Journal or Publication Title: The Journal of Cell Biology
Volume: 121
Number: 4
Date Deposited: 17 Jul 2008 17:08
Date: 1993
Page Range: pp. 743-750
Faculties / Institutes: Service facilities > Center for Molecular Biology Heidelberg
DDC-classification: 570 Life sciences
Series: Works by Bernhard Dobberstein
About | FAQ | Contact | Imprint |
OA-LogoDINI certificate 2013Logo der Open-Archives-Initiative